4×sds page loading buffer (Thermo Fisher)

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Thermo Fisher 4×sds page loading buffer
4×Sds Page Loading Buffer, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/sds-page+sample+loading+buffer+4/Laemmli+SDS+sample+buffer%2C+reducing/us07041644-243-6-36
Average 99 stars, based on 1 article reviews

4×sds page loading buffer - by Bioz Stars, 2026-11

99/100 stars

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Nucleic Acid Electrophoresis:

Article Title: The kinase defective EPHB6 receptor tyrosine kinase activates MAP kinase signaling in lung adenocarcinoma.
Article Snippet: .. Cell lysates were cleared at 20,000 g for 10 min. After adjustment of protein concentrations, the lysates were heated in SDS sample loading buffer at 72 ̊C for 10 min and separated by SDS-polyacrylamide gel electrophoresis (PAGE, 4-12%, Invitrogen). .. Gels were blotted on a polyvinylidene difluoride (PVDF) membrane (Immobilon P, Millipore, Bedford, MA, USA) and stained with the indicated first antibody [anti-human EPHB6 (1 μg/ml, From Santa Cruz Inc., Santa Cruz, CA, USA or ABGENT, San Diego, CA, USA), anti-human phosphorylated or total ERK (1:1000 dilution, from Cell Signaling Technology, Inc., Danvers, MA, USA), and antihuman ß-actin (40 ng/ml, Santa Cruz Inc.).

Polyacrylamide Gel Electrophoresis:

SDS Page:

Article Title: Differential recruitment efficacy of patient-derived amyloidogenic and myeloma light chain proteins by synthetic fibrils—A metric for predicting amyloid propensity
Article Snippet: .. Samples were then rotated for 4 days at RT after which time 20 μL of sample was mixed with 7.5 μL SDS-PAGE loading buffer, boiled for 10 min and loaded onto 4–12% Bis-Tris SDS-PAGE gels (Invitrogen). .. The SDS-PAGE gel was then analyzed by a phosphor imager (Cyclone Storage Phosphor System; PerkinElmer) after 2 h exposure.

Article Title: Structural basis of human LRRK2 membrane recruitment and activation
Article Snippet: .. Briefly, cell lysates were mixed with a quarter of a volume of 4 x SDS-PAGE loading buffer (Invitrogen™ NuPAGE™ LDS Sample Buffer, cat# NP0007) and heated at 70 °C for 5 min. .. Samples were loaded onto NuPAGE 4–12% Bis–Tris Midi Gels (Thermo Fisher Scientific, Cat# WG1402BOX or Cat# WG1403BOX) and electrophoresed at 130 V for 2 hrs in NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific, Cat# NP0001-02).

Article Title: GDF11 Regulates PC12 Neural Stem Cells via ALK5-Dependent PI3K-Akt Signaling Pathway.
Article Snippet: Total protein was measured by using a BCA protein assay kit (Beyotime Biotechnology, China). .. Then, equal amount proteins (25 μg) of each sample were denatured with NuPAGE 4× LDS loading buffer (Invitrogen, USA), and were separated by SDS-PAGE (NuPAGE 4–12% Bis-Tris gel; Invitrogen, USA). .. After transferred to PVDF membranes, proteins were incubated with the following rabbit antibodies at 4 ◦C overnight: anti-nestin (Cell Signal Technology, Danvers, MA, USA), anti-βIII-tubulin (Cell Signal Technology, USA), anti-GFAP (Cell Signal Technology, USA), and anti-GAPDH (Cell Signal Technology, USA), anti-BCL-2 (Cell Signal Technology, USA), anti-Bax (Cell Signal Technology, USA), anti-Cleaved caspase-3 (Cell Signal Technology, USA), anti-Smad2/3 (Cell Signal Technology, USA), anti-PI3K p85 (Cell Signal Technology, USA), anti-Akt (Cell Signal Technology, USA), anti-p-Smad2/3 Int.

Article Title: GDF11 Regulates PC12 Neural Stem Cells via ALK5-Dependent PI3K-Akt Signaling Pathway
Article Snippet: Total protein was measured by using a BCA protein assay kit (Beyotime Biotechnology, China). .. Then, equal amount proteins (25 μg) of each sample were denatured with NuPAGE 4× LDS loading buffer (Invitrogen, USA), and were separated by SDS-PAGE (NuPAGE 4–12% Bis-Tris gel; Invitrogen, USA). .. After transferred to PVDF membranes, proteins were incubated with the following rabbit antibodies at 4 °C overnight: anti-nestin (Cell Signal Technology, Danvers, MA, USA), anti-βIII-tubulin (Cell Signal Technology, USA), anti-GFAP (Cell Signal Technology, USA), and anti-GAPDH (Cell Signal Technology, USA), anti-BCL-2 (Cell Signal Technology, USA), anti-Bax (Cell Signal Technology, USA), anti-Cleaved caspase-3 (Cell Signal Technology, USA), anti-Smad2/3 (Cell Signal Technology, USA), anti-PI3K p85 (Cell Signal Technology, USA), anti-Akt (Cell Signal Technology, USA), anti-p-Smad2/3 (Cell Signal Technology, USA), anti-p-PI3K p85 (Cell Signal Technology, USA), anti-p-Akt (Cell Signal Technology, USA), and β-actin (Cell Signal Technology, USA), respectively.

Article Title: The CD22-IGF2R interaction is a therapeutic target for microglial lysosome dysfunction in Niemann-Pick type C
Article Snippet: The supernatant was collected, and protein concentration was measured with the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). .. An aliquot containing 20 μg of protein from each sample was mixed with 4× loading buffer (Thermo Fisher Scientific), 2.5% beta-mercaptoethanol (MilliporeSigma), and boiled for 5 min at 95°C before being subjected to SDS-PAGE and transferred to a nitrocellulose membrane (Bio-Rad). .. The membrane was first stained for total protein with Revert (LI-COR).

Article Title: Neurotoxic tau oligomers after single versus repetitive mild traumatic brain injury
Article Snippet: .. The reaction of the enzyme was stopped by adding 4× SDS-PAGE loading buffer (NuPAGE LDS sample buffer, Invitrogen). .. The reaction of the enzyme was stopped by adding 4× SDS-PAGE loading buffer (NuPAGE LDS sample buffer, Invitrogen).

Membrane:

other:

Article Title: Yeast transcription factor Msn2 binds to G4 DNA.
Article Snippet: Whole cell lysates wer e r esuspended in 2 × SDS sample buffer and boiled for 5 min at 95 ◦C before loading onto 4–20% SDS-PAGE gels (Thermo Fisher Scientific, Cat# XP04202BOX).

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